Table 2.

Final concentration of reagent B used to measure HNA

	Volume (µl)	Reagent	Final concentration
Sample	4
R1	180	Succinate buffer (pH 4.8)	123 (mM)
		Triton X-405	0.2 (%)
R2	90	Succinate buffer (pH 4.8)	123 (mM)
		BCP	150 (µM)
		Triton X-405	0.2 (%)