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Competition association binding of mGlu5 NAMs at HEK293A-mGlu5-low membranes. [3H]methoxy-PEPy specific binding measured at different time points in the absence or presence of indicated concentrations of different NAMs added simultaneously. Data are means ± S.E.M. from three to eight independent experiments performed in duplicate. Error bars not shown lie within the dimensions of the symbol. Curves shown are the best fit of the entire data set with eq. 4.
