Fig. 3.

Inhibition of l-glutamate–induced signaling in HEK293A-mGlu₅-low cells. Effect of NAMs on Ca²⁺ mobilization response to 320 nM l-glutamate (A), IP₁ accumulation in response to 3.2 μM l-glutamate (B), and ERK1/2 phosphorylation measured after 5-minute incubation with 3.2 μM l-glutamate (C). Data are means + S.E.M. from three to five independent experiments performed in triplicate. Error bars not shown lie within the dimensions of the symbol. Data were normalized to 0% as the mean of the vehicle response and 100% as the mean of maximal l-glutamate response.