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. 2020 Nov 17;11:601555. doi: 10.3389/fmicb.2020.601555

Figure 1.

Figure 1

Selective inhibition of plasmid-encoded gene expression by I24. (A) HEK293 cells were transfected with an expression plasmid encoding firefly luciferase under control of the CMV promoter in the presence of increasing concentrations of peptide I24. Results shown are luciferase levels (mean ± SD from triplicate wells). (B) HEK293 cells were transfected with a truncated IL-8 (CXCL8) promoter-luciferase expression construct in the presence of increasing concentrations of I24 and treated with TNF-α 6 h after transfection for further 18 h. Luciferase was determined from cell lysates and supernatants analyzed for CXCL8 by ELISA. Results shown are mean ± SD from duplicate wells expressed as % of vehicle-treated control. (C) Total RNA was isolated from cells transfected with the luciferase expression plasmid and stimulated with TNF-α 6 h after transfection for further 18 h before isolation of total RNA. Results shown are luciferase and CXCL8 mRNA levels normalized to GAPDH mRNA expressed as % of vehicle-treated control. (D) HEK293 cells were transfected with the luciferase expression plasmid and increasing concentrations of I24, TAT (47–57), or TAT-I24. Luciferase levels (mean ± SD from duplicate wells) expressed as % of vehicle-treated cells are shown.