Figure 4.

Inhibition of MCMV replication by TAT-I24. (A) NIH/3T3 cells were treated with increasing concentrations of TAT (48–60) or TAT-I24 and infected with murine CMV virus expressing firefly luciferase at a MOI of 0.5. After 72 h, luciferase was determined in the cell lysates. Results shown are mean ± SD from duplicate wells expressed as % of vehicle control. (B) Viable cells (fluorescence) and dead cells (luminescence; RLU = relative light units) in the presence of increasing concentrations of TAT or TAT-I24 determined by the cytotoxicity assay in triplicates. As positive control, 100% lysed cells are shown. (C) Microscopic examination (20 × objective) of NIH/3T3 cells infected with MCMV S-mCherry-SCP in the presence of increasing concentrations of TAT (48–60) or TAT-I24. Nuclei were stained with DAPI. Scale bars indicate 100 μm.