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. 2020 Nov 17;11:601555. doi: 10.3389/fmicb.2020.601555

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Copyright © 2020 Ruzsics, Hoffmann, Riedl, Krawczyk, Widera, Sertznig, Schipper, Kapper-Falcone, Debreczeny, Ernst, Grabherr, Hengel and Harant.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Inhibition of HCMV replication by TAT-I24. (A) MRC-5 cells were transduced with recombinant reporter construct AAV-GLuc-3B and treated on the next day with TAT (48–60) or TAT-I24 and infected with the HCMV strain TB40 at a MOI of 0.2. Results shown are Gaussia luciferase levels in the supernatants (mean ± SD from triplicate wells) 3 days after infection expressed as % of vehicle-treated cells. (B) Total RNA was isolated from cells from the replicon assay. Results shown are UL44 mRNA levels normalized to GAPDH mRNA levels expressed as % of control.