TABLE 3.

Core genes involved with fengycin and surfactin in B. velezensis HAB-2, B. velezensis FZB42, B. amyloliquefaciens DSM7, and B. subtilis 168.

Compound	Gene	Presence (+) or absence (−)				Functions or product
		HAB-2	FZB42	DSM7	168
Fengycin	dacC	+	+	+	+	DacC protein
	fenA	+	+	+	+	Fengycin synthetase A, scaffold biosynthesis
	fenB	+	+	−	+	Fengycin synthetase B, scaffold biosynthesis
	fenC	+	+	−	+	Fengycin synthetase C, scaffold biosynthesis
	fenD	+	+	−	+	Fengycin synthetase D, scaffold biosynthesis
	fenE	+	+	+	+	Fengycin synthetase E, scaffold biosynthesis
Surfactin	yx01	+	+	+	−	Yx01 protein, alcohol dehydrogenase
	yckE	−	+	+	+	YckE protein
	srfAA	+	+	+	+	Surfactin synthetase A, scaffold biosynthesis
	srfAB	+	+	+	+	Surfactin synthetase B, scaffold biosynthesis
	srfAC	+	+	+	+	Surfactin synthetase C, scaffold biosynthesis
	srfAD	+	+	+	+	Surfactin synthetase D, other enzymatic
	aat	+	+	+	+	Amino transferase
	ycxC	+	+	−	+	Transporter
	ycxD	+	+	−	+	Transcriptional regulator containing an aminotransferase domain
	sfp	+	+	+	+	Phosphopantetheinyl transferase involved in non-ribosomal synthesis
	yczE	+	+	+	+	Integral membrane protein involved in non-ribosomal synthesis
	yckI	+	+	+	+	YckI protein
	yckJ	+	+	+	+	YckJ protein

Secondary metabolite antibacterial gene clusters of the four strains were compared using anti-SMASH 4.1.0 (Weber et al., 2015), reference gene clusters and core genes analyzed with MIBiG (Minimum Information about a Biosynthetic Gene cluster) (Kautsar et al., 2020).