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. 2020 Nov 27;11:2041731420967791. doi: 10.1177/2041731420967791

Figure 5.

Figure 5.

Osteogenesis differentiation of BMSCs in vitro. (a) Alizarin red staining for calcium deposition at 14 d (Scale bar: 200 µm). (b) ALP staining at week 1 and week 2 (Scale bar: 200 µm). (c) Mmunofluorescence assays for OCN expression at 21 d, with the nucleus stained with DAPI and OCN stained in green (Scale bar: 100 µm). The corresponding quantitative analysis of Alizarin red staining for calcium deposition (d), ALP at 1 week and 2 weeks (e), ALP activity of BMSCs after 3, 7, and 14 days (f) and OCN protein expression (g).

*p < 0.05, **p < 0.01, ***p < 0.001, compared with the Control group; ###p < 0.001, compared with the SFFHM group, (d) n = 6, (e) n = 3, (f) n = 5, (g) n = 4, ns = not significant.