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. 2020 Nov 27;11:2041731420967791. doi: 10.1177/2041731420967791

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© The Author(s) 2020

This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access page (https://us.sagepub.com/en-us/nam/open-access-at-sage).

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Figure 8. — Immunostaining of bone tissue specimens at 4 and 8 weeks: (a and c) Immunofluorescence images of Col-I expression and quantitative analysis for tissue sections after scaffolds implantation 4 weeks (green: OCN; blue: DAPI), (b) Immunohistochemical images of ALP, Col-I and OCN expression, scale bar = 100 µm (400×). (d–f) Quantitative analysis of new bone formation expressed as ALP, Col-I and OCN levels.

***p < 0.001, compared with Control group; ^#p < 0.05, ^###p < 0.001, compared with SFFHM group; n = 4, ns = not significant).