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. 2020 Nov 17;10:532581. doi: 10.3389/fonc.2020.532581

Figure 5.

Figure 5

LINC01089 functioned as a ceRNA for miR-27a in NSCLC. (A) Subcellular localization of LINC01089 was detected through nucleocytoplasmic separation assay. (B) A schematic outline of sequence sites of miR-27a targeted to LINC01089 predicted by bioinformatic analysis. (C) The association between LINC01089 and miR-27a was evaluated by Ago2-RIP assay. RNA immunoprecipitation (RIP) experiments were performed in A549 cells overexpressing miR-27a or miR-NC. (D) RNA pull-down assay was used to detect the direct interaction between miR-27a and LINC01089. (E) Dual-luciferase reporter assay was carried out to examine the luciferase activity of LINC01089-wt group and LINC01089-mut group. (F) The relationship between LINC01089 expression and miR-27a expression was examined by Spearman correlation analysis in 60 NSCLC tissues. (G) The expression of miR-27a was detected in siLINC01089 transfected A549 and H1299 cells. Data are reported as means ± SD. **P < 0.01; ***P < 0.001.