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. 2020 Nov 17;10:532581. doi: 10.3389/fonc.2020.532581

Figure 7.

Figure 7

LINC01089 suppresses EMT of NSCLC via miR-27a-SFRP1-Wnt/β-catenin axis. (A) miR-27a expression was validated in A549 cells transfected with inhibitor and/or siLINC01089 by RT-qPCR (B, C) A549 cells were transfected with miR-27a mimics and/or siLINC01089, CCK-8 and transwell assays were to detect the proliferation viability and migration capability. (D) Quantification of C (E) Spearman correlation was used to analyze the correlation between LINC01089 and SFRP1 expression in 60 NSCLC tissues. (F) RT-qPCR was used to analyze the mRNA levels of N-cadherin, Snail, Slug and SRPR1 in A549 cells cotransfected with miR-27a inhibitor and/or siLINC01089. (G) Western blot was used to analyze the protein levels of p-GSK-3P and β-catenin in A549 cells cotransfected with miR-27a inhibitor and/or siLINC01089. GAPDH acted as reference gene. (H) RT-qPCR was used to analyze the mRNA levels of N-cadherin, E-cadherin, Snail, Slug, and SRPR1 in PC9 cells cotransfected with pcDNA3.1-LINC01089 and/or siSFRP1. (I) Western blot was used to analyze the protein levels of p-GSK-3P and β-catenin in PC9 cells cotransfected with pcDNA3.1-LINC01089 and/or siSFRP1. GAPDH acted as reference gene. Data are reported as means ± SD. *P < 0.05; **P < 0.01; ***P < 0.001.