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. 2020 Nov 17;10:532581. doi: 10.3389/fonc.2020.532581

Table 1.

Primer sequences of the genes used for RT-qPCR.

Gene Forward 5′-3′ Reverse 5′-3′
GAPDH GGTGAAGGTCGGAGTCAACG ACCATGTAGTTGAGGTCAATGA
E-cadherin TTCAAAGTGGGCACAGATGGT TAGGTGGAGTCCCAGGCGTA
N-cadherin AAGGCGTTATGTGTGTATCTTC TGGAAAGCTTCTCACGGCAT
Snail CTTCGCTGACCGCTCCAACC GGAGCAGGGACATTCGGGAG
Slug
LINC01089
SFRP1
miR-27a
GGCTCATCTGCAGACCCATT
AGTCAGGAGCCCTCCTATGG
ATGATGATGACAACGACATA
TTCACAGTGGCTAAG
TGCTACACAGCAGCCAGATT
GGGAGCCAAGGCACTCTAAG
ATGCGCTTGAACTCTCTCTGC
GTGCAGGGTCCGAGGT
The sequences of the siRNAs and miRNAs used in transfection.
siRNA Sense 5′-3′
siLINC01089-1 GUCAGGAGCCCUCCUAUGG
siLINC01089-2 GAACGUGAGGGUGUAACUU
siSFRP1 UGAUGAUGACAACGACAUA
siNC
miR-27a mimic
miR-27a inhibitor
MiR-NC
ACGUGACACGUUCGGAGAA
UUCACAGUGGCUAAGUUCCGC
GCGGAACUUAGCCCACUGUGAA
UUGUACUACACAAAAGUACUG
The sequences of the shRNAs.
shRNA Sense 5'-3'
shLINC01089-1 GTCAGGAGCCCTCCTATGGGC
shLINC01089-2 GAACGTGAGGGTGTAACTTAC
shNC ACGTGACACGTTCGGAGAAA