Table 1.
Primer sequences of the genes used for RT-qPCR.
| Gene | Forward 5′-3′ | Reverse 5′-3′ |
|---|---|---|
| GAPDH | GGTGAAGGTCGGAGTCAACG | ACCATGTAGTTGAGGTCAATGA |
| E-cadherin | TTCAAAGTGGGCACAGATGGT | TAGGTGGAGTCCCAGGCGTA |
| N-cadherin | AAGGCGTTATGTGTGTATCTTC | TGGAAAGCTTCTCACGGCAT |
| Snail | CTTCGCTGACCGCTCCAACC | GGAGCAGGGACATTCGGGAG |
|
Slug LINC01089 SFRP1 miR-27a |
GGCTCATCTGCAGACCCATT AGTCAGGAGCCCTCCTATGG ATGATGATGACAACGACATA TTCACAGTGGCTAAG |
TGCTACACAGCAGCCAGATT GGGAGCCAAGGCACTCTAAG ATGCGCTTGAACTCTCTCTGC GTGCAGGGTCCGAGGT |
| The sequences of the siRNAs and miRNAs used in transfection. | ||
| siRNA | Sense 5′-3′ | |
| siLINC01089-1 | GUCAGGAGCCCUCCUAUGG | |
| siLINC01089-2 | GAACGUGAGGGUGUAACUU | |
| siSFRP1 | UGAUGAUGACAACGACAUA | |
|
siNC miR-27a mimic miR-27a inhibitor MiR-NC |
ACGUGACACGUUCGGAGAA UUCACAGUGGCUAAGUUCCGC GCGGAACUUAGCCCACUGUGAA UUGUACUACACAAAAGUACUG |
|
| The sequences of the shRNAs. | ||
| shRNA | Sense 5'-3' | |
| shLINC01089-1 | GTCAGGAGCCCTCCTATGGGC | |
| shLINC01089-2 | GAACGTGAGGGTGTAACTTAC | |
| shNC | ACGTGACACGTTCGGAGAAA | |