Table 3.
Genomic Changes of K2606 After Exposure to Tigecycline
| Location | Mutation | Position | Mutation Type | Information | Annotation | Gene Description |
|---|---|---|---|---|---|---|
| SNPa in both K2606-4 and K2606-16 | ||||||
| Chrb | T843830G | Intergenic of gene0838 and gene0839 | - | - | fimB;- | type 1 fimbriae regulatory protein; hypothetical protein |
| Chr | A1127404G, A1127631G A1127769G, A1128074G |
Intergenic of gene1117 and gene1123 | - | - | csrA;- | carbon storage regulator; phospholipid-binding domain protein |
| Chr | C3872385 T | Intergenic of gene3810 and gene3811 | - | - |
romA; ramR |
beta-lactamase domain protein; TetR family transcriptional regulator |
| pAc | A90269C | Internal of gene0095 | Nonsynonymous | c.T751G p.S251A |
tetA | tetracycline efflux MFS transporter |
| SNP in K2606-4 only | ||||||
| Chr | G303519A | Intergenic of gene0279 and gene0278 | - | - | tusA;- | tRNA 2-thiouridine (34) synthase; hypothetical protein |
| Chr | G586814C | Intergenic of gene0574 and gene0575 | - | -;- | hypothetical protein; hypothetical protein |
|
| Chr | G4324175T | Internal of gene4263 | Nonsynonymous | c.G386T, p.R129L | dksA | transcriptional regulator |
| Chr | C4350054A | Internal of gene4291 | Nonsynonymous | c.G59T, p.C20F | - | hypothetical protein |
| pBd | C125391G | Intergenic of gene0112 and gene0113 | - | -;higA | hypothetical protein; transcriptional regulator |
|
| SNP in K2606-16 only | ||||||
| Chr | C427705A | gene0395 | Nonsynonymous | c. C116A, p.P39Q |
rpsJ | ribosome protein RpsJ |
| Chr | C2049592G | Intergenic of gene1998 and gene1999 | - | - | -;- | GNAT family acetyltransferase; transcriptional regulator |
| Chr | A4052373C | Internal of gene3986 | Nonsynonymous | c. A101C, p.Y34S, |
- | hypothetical protein |
| pDe | C29141G | Intergenic of gene0036 and gene0037 | - | - | -;- | hypothetical protein; hypothetical protein |
| fINDEL in K2606-4 | ||||||
| Chr | GCTATACCAAA deleted between 586,798 and 586,808 | Intergenic of gene0572 and gene0573 | Deletion | - | glxK;- | glycerate 2-kinase; hypothetical protein |
Notes: aSNP, single nucleotide polymorphism; bChr, chromosome; cpA, plasmid A; dpB, plasmid B; epD, plasmid D; fINDEL, insertion-deletion.