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. 2020 Nov 26;13:12213–12223. doi: 10.2147/OTT.S284219

Figure 2.

Figure 2

PC4 knockdown inhibited cell proliferation and arrested the cell cycle at the G1-S transition in vitro. PC4 protein (A) and mRNA (B) expression levels in PDAC cell lines (PANC-1, AsPC-1 and CFPAC-1) and normal human pancreatic duct epithelial cells (hTERT-HPNE). PC4 protein (C) and mRNA (D) expression levels in cells infected with either lentivirus expressing shRNA targeting PC4 (shPC4-1 and shPC4-2) or control empty lentivirus. (E) Cell viability of AsPC-1 and CFPAC-1 cells was measured using a CCK-8 assay 24 h, 48 h, 72 h, 96 h and 120 h after PC4 knockdown. (F, G) Cell cycle distribution in AsPC-1 and CFPAC-1 cells with stable PC4 knockdown and corresponding controls was determined by flow cytometry. Experiments were repeated three times independently. (H, I) Apoptosis rate in AsPC-1 and CFPAC-1 cells with stable PC4 knockdown and corresponding controls was determined by flow cytometry. Experiments were repeated three times independently. All data represent the means ± SDs. **P<0.01,*** P<0.001, **** P<0.0001.