Figure 5.

MRAP2 forms higher-order oligomers. A, HEK293T cells transfected with FLAG-MRAP2 were incubated with an irreversible cross-linker (DSG or DSS). Proteins in cell lysate were separated by SDS-PAGE and immunoblotted. B, HEK293T cells expressing FLAG-MRAP2 were solubilized with varying concentrations of DDM and analyzed by blue native PAGE and immunoblotted. The band of ∼66 kDa is the approximately the molecular mass of a FLAG-MRAP2 dimer. C, schematic showing possible MRAP2 higher-order oligomers. WB, Western blotting.