Figure 5.

Structure of a 14-3-3 diphosphopeptide complex. A, surface representation of the 14-3-3 dimer (monomers containing the pSer¹⁰⁰ and pSer⁵¹¹ binding sites are shown in green and gray, respectively) with secondary structure shown in ribbons. The pSer¹⁰⁰ and pSer⁵¹¹ ends of the diphosphorylated peptide are shown as sticks in yellow. B, stereo image of difference maps of the pSer¹⁰⁰ binding site. C, stereo image of difference maps of the pSer⁵¹¹ binding site. The parallel lines represent the parallel stacking interactions between Arg⁵⁰⁸ on the diphosphopeptide and Arg⁶⁰ on 14-3-3.