Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Aug 3;295(48):16207–16216. doi: 10.1074/jbc.RA120.013899

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2020 Smith et al.

Published under exclusive license by The American Society for Biochemistry and Molecular Biology, Inc.

PMC Copyright notice

Figure 4. — Flux through NNT contributes to energy expenditure during ΔG_ATP clamped respiration. A, JO₂ versus clamped free energy of ATP hydrolysis (ΔG_ATP; increasing ATP/ADP free concentration ratio) in skeletal muscle mitochondria isolated from C57BL/6N (which express NNT) and C57BL/6J (which do not express NNT), supported by PCoA (20 μm) and carnitine (5 mm) −/+ BCNU/auranofin (0.1 μm/100 μm). B, ΔΨ_m versus clamped free energy of ATP hydrolysis (ΔG_ATP) measured under the same conditions. C, JH₂O₂ versus clamped free energy of ATP hydrolysis (ΔG_ATP) measured under the same conditions. D, pairwise comparison of JO₂ between groups from A at the most negative ΔG_ATP (highest ATP/ADP ratio). E, pairwise comparison of ΔΨ_m between groups from A at the most negative ΔG_ATP. F, pairwise comparison of JH₂O₂ between groups from A at the most negative ΔG_ATP. All data are means ± S.E. (error bars); *, p < 0.05 compared with B6N using two-way ANOVA (repeated measures for A–C); n = 8 mice/group (JO₂/JH₂O₂); n = 4 mice/group (ΔΨ_m).