Table 3.
Three modeling fit searches for FHR5 using X-ray scattering curve fits and sedimentation coefficients
| Search | Filter | Modelsa | Rgb | Rgb | Rxs | Dmax | R-factor | s20,w |
|---|---|---|---|---|---|---|---|---|
| nm | nm | nm | nm | % | S | |||
| Search 1 (all linkers 2–8) | None | 86,732 | 4.42–8.29 | NA | 0.01–4.23 | NA | 4.46–27.14 | NA |
| Tris-150 0.17 mg/ml | R-factor | 100 | 5.24–6.02 | NA | 2.00–2.73 | NA | 4.46–5.80 | NA |
| R-factor, Rg, Rxs | 28 | 5.24–5.54 | NA | 2.25–2.48 | NA | 4.46–5.39 | NA | |
| Best fit | 1 | 5.45 | 5.67 | 2.25 | 20.0 | 4.46 | 5.2 | |
| Experimental | NA | 5.44 | 5.72 | 2.39 | 20.0 | NA | NA | |
| Tris-150 0.5 mg/ml | R-factor | 100 | 5.24–5.84 | NA | 2.33–2.65 | NA | 4.21–5.30 | NA |
| R-factor, Rg, Rxs | 131 | 5.28–5.79 | NA | 2.33–2.57 | NA | 4.21–5.30 | NA | |
| Best fit | 1 | 5.31 | 5.63 | 2.44 | 20.0 | 4.21–5.98 | 5.2 | |
| Experimental | NA | 5.52 | 5.85 | 2.45 | 21.0 | NA | NA | |
| Search 2 (linkers 2, 4, 6, 7) | None | 72,755 | 4.52–8.66 | NA | 0.01–4.66 | NA | 4.68–30.89 | NA |
| Tris-150 0.17 mg/ml | R-factor | 100 | 5.01–5.88 | NA | 2.19–2.71 | NA | 4.68–6.01 | NA |
| R-factor, Rg, Rxs | 55 | 5.18–5.70 | NA | 2.27–2.51 | NA | 4.68–5.84 | NA | |
| Best fit | 1 | 5.45 | 5.89 | 2.37 | 20.0 | 4.68 | 5.2 | |
| Experimental | NA | 5.44 | 5.72 | 2.39 | 20.0 | NA | NA | |
| Tris-150 0.5 mg/ml | R-factor | 100 | 5.01–6.02 | NA | 2.19–2.82 | NA | 3.86–6.15 | NA |
| R-factor, Rg, Rxs | 52 | 5.25–5.74 | NA | 2.57–2.34 | NA | 3.86–5.99 | NA | |
| Best fit | 1 | 5.25 | 5.38 | 2.48 | 20.0 | 3.86 | 5.5 | |
| Experimental | NA | 5.52 | 5.85 | 2.45 | 21.0 | NA | NA | |
| Search 3 (linkers 3 and 6) | None | 123,776 | 4.54–7.89 | NA | 0.004–4.57 | NA | 4.28–28.14 | NA |
| Tris-150 0.17 mg/ml | R-factor | 100 | 5.16–5.69 | NA | 2.14–2.53 | NA | 4.28–4.61 | NA |
| R-factor, Rg, Rxs | 694 | 5.17–5.69 | NA | 2.27–2.51 | NA | 4.28–6.00 | NA | |
| Best fit | 1 | 5.36 | 5.58 | 2.37 | 20.0 | 4.28 | 5.4 | |
| Experimental | NA | 5.44 | 5.72 | 2.39 | 20.0 | NA | NA | |
| Tris-150 0.5 mg/ml | R-factor | 100 | 5.13–5.66 | NA | 2.22–2.69 | NA | 3.84–4.65 | NA |
| R-factor, Rg, Rxs | 749 | 5.26–5.79 | NA | 2.33–2.57 | NA | 3.84–5.99 | NA | |
| Best fit | 1 | 5.34 | 5.64 | 2.52 | 20.0 | 3.84 | 5.1 | |
| Experimental | NA | 5.52 | 5.85 | 2.45 | 21.0 | NA | NA |
a Total number of models accepted after Monte Carlo simulations and after model filtering. The best fit model corresponds to that with the lowest R-factor in the filtered models.
b The first Rg value of the pair is from Guinier analyses, and the second is from the P(r) analyses. NA, not available.