Skip to main content
. 2020 Sep 14;295(48):16267–16279. doi: 10.1074/jbc.RA120.014591

Figure 2.

Figure 2.

N-MADD-4B undergoes processing. A, SDS-PAGE analysis of peak elution fraction from affinity chromatography (Protein A, data not shown) evidenced intact N-MADD-4B (apparent mass, ∼45 kDa) and N-MADD-4B fragments (≤35 kDa). The protein identity in each band was confirmed by MS (see Fig. S1). B, cation-exchange chromatography (MonoS, NaCl gradient) resolves the N-MADD-4B fragments (elution volume, 5–6 ml) from intact N-MADD-4B (12–13 ml). Inset, SDS-PAGE analysis of the peak elution fractions. C, SEC (Superdex-75 10/300) does not resolve intact N-MADD-4B (solid line) from the fragments (dashed line). Inset, SDS-PAGE analysis of the peak elution fractions.