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. 2020 Sep 14;295(48):16267–16279. doi: 10.1074/jbc.RA120.014591

Figure 6.

Figure 6.

N-MADD-4B rescues GABAAR clustering defect in the C. elegans madd-4 null mutant. A, confocal detection of RFP-GABAAR expressed from the unc-49::rfp knock-in locus kr296 in WT or madd-4(kr270) null mutant animals (magenta). B, N-MADD-4B-GFP or TSP1–4-GFP were expressed in GABAergic neurons of madd-4(kr270) mutant animals. The MADD-4B(*)-GFP row corresponds to N-MADD-4B-GFP (left panels) or TSP1–4-GFP (right panels). B′, functional domains of MADD-4B, N-MADD-4B, and TSP1–4. Gray, signal peptide; red, T7 tag; purple, PLAC domain. C, RFP-GABAAR fluorescence levels were quantified in WT, madd-4(kr270), N-MADD-4B, and TSP1–4 rescue groups and normalized to the WT group (mean ± S.E. (error bars)). One-way analysis of variance was used, followed by Tukey's multiple-comparison test. ***, p < 0.001; n.s, not significant. The numbers of animals are indicated inside the histogram boxes. Scale bar, 10 μm.