Table 2.
Fit parameters obtained for MST experiments between N-MADD-4B and labeled NLG-1 (NLG*) or NLG-1 and labeled Ig (Ig*)
The N-MADD-4B/NLG* binding data (Fig. 4A), N-MADD-4B/NLG*/NLG-1 competition data (Fig. 4B), and NLG-1/Ig* binding data (Fig. 4C) were processed using either the Kd or the Hill model (see “Experimental procedures”).
| Fit parameters | Kd model | Hill model |
|---|---|---|
| N-MADD-4B/ NLG* | ||
| Kd or EC50 (nm) | 465 | 578 |
| Kd confidence (nm) | ±130 | ±77 |
| S.E. (nm) | 1.3 | 1.2 |
| Reduced χ2 | 1.6 | 0.8 |
| Signal/noise | 14.9 | 13.1 |
| Hill coefficient | 2.3 | |
| N-MADD-4B/NLG*/NLG-1 | ||
| Kd or EC50 (nm) | 1428 | 1396 |
| Kd confidence (nm) | ±488 | ±285 |
| S.E. (nm) | 0.8 | 0.8 |
| Reduced χ2 | 1.1 | 1.1 |
| Signal/noise | 17.8 | 17.5 |
| Hill coefficient | 1.0 | |
| NLG-1/Ig* | ||
| Kd or EC50 (nm) | 6139 | 5580 |
| Kd confidence (nm) | ±1220 | ±825 |
| S.E. (nm) | 1.4 | 1.3 |
| Reduced χ2 | 0.5 | 0.2 |
| Signal/noise | 16.1 | 16.3 |
| Hill coefficient | 1.4 |