Table 3.
Fit parameters obtained for N-MADD-4B binding to labeled NLG-1 (NLG*) in the presence or absence of heparin
The N-MADD-4B/NLG* binding data from experiments performed in the absence or presence of heparin (Fig. 5A) and the N-MADD-4B/NLG*/heparin competition data (Fig. 5B) were processed using the Hill model (see “Experimental procedures”).
| Fit parameters | Binding without heparin | Binding with heparin | Competition experiment |
|---|---|---|---|
| Kd or EC50 (nm) | 467 | 1782 | 740 |
| Kd confidence (nm) | ±31 | ±483 | ±136 |
| S.E. (nm) | 0.9 | 0.8 | 1.0 |
| Reduced χ2 | 0.6 | 1.0 | 6.9 |
| Signal/noise | 14.5 | 17.1 | 6.7 |
| Hill coefficient | 4.3 | 1.1 | 3.0 |