Figure 1.
Cross-species deacylation of Hs and Cc ProXp-ala. A, predicted secondary structures of Hs cyto, mito, and Ec tRNAPro. Arrows indicate the acceptor-stem positions mutated in this study. B, deacylation of Hs cyto, mito, and Ec Ala-tRNAPro by Hs ProXp-ala. C, deacylation of Hs cyto, mito, and Ec Ala-tRNAPro by Cc ProXp-ala. Representative time courses of three independent experiments are shown. The reactions were performed with 1.5 μm Hs ProXp-ala and 100 nm Ala-tRNAPro at 30 °C (B) or with 0.75 μm Cc ProXp-ala and 100 nm Ala-tRNAPro at 18 °C (C), as described under “Experimental procedures.”