Figure 3.
Deacylation of Ala-tRNAPro acceptor-stem mutants by Hs ProXp-ala. Hs tRNAPro mutants (open circles) and Ec tRNAPro variants (filled circles). The same color was chosen for tRNAPro mutants with identical nucleotides at positions 1:72 and 73: Hs WT and Ec G1:C72/C73 (red), Hs A73 and Ec G1:C72 (green), Hs C1:G72 and Ec C73 (blue), and Hs C1:G72/A73 and Ec WT (magenta). The reactions were performed with 1.5 μm Hs ProXp-ala and 100 nm Ala-tRNAPro at 30 °C. Representative time courses of three independent experiments are shown.