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. 2020 Oct 13;295(48):16180–16190. doi: 10.1074/jbc.RA120.015981

Figure 7.

Figure 7.

Deacylation of Ala- and Pro-tRNAPro by WT and mutant ProXp-ala. A, deacylation of Hs tRNAPro charged with Ala (red) and Pro (cyan) by WT (circles) and H137A (triangles) Hs ProXp-ala. B, deacylation of Ec tRNAPro charged with Ala (red) and Pro (cyan) by WT (circles) and H130A (triangles) Cc ProXp-ala. The reactions were performed with 1.5 μm Hs ProXp-ala and 100 nm Hs aminoacyl-tRNAPro at 30 °C or with 0.75 μm Cc ProXp-ala and 100 nm Ec aminoacyl-tRNAPro at 18 °C. Representative time courses of three independent experiments are shown.