Table 2.
Rate constants (kobs) for deacylation of Hs aa-tRNAPro by Hs ProXp-ala
Deacylation assays were performed at 30 °C using 1.5 μm Hs ProXp-ala and 100 nm aa-tRNAPro. Fold change is reported relative to Hs Ala-tRNAPro. The results are the averages of three independent trials with the standard deviation indicated.
| Aminoacyl-tRNAPro | Amino acid volume | kobs | Fold change |
|---|---|---|---|
| Å3 | min−1 | ||
| Ala-tRNAPro | 101 | 0.79 ± 0.12 | |
| Abu-tRNAPro | 122 | 0.039 ± 0.007 | 20 |
| Ser-tRNAPro | 110 | 0.039 ± 0.003 | 20 |
| Thr-tRNAPro | 129 | 0.015 ± 0.002 | 53 |
| Aze-tRNAPro | 117 | 0.23 ± 0.026 | 3.4 |
| Pro-tRNAPro | 132 | 0.16 ± 0.012 | 5.0 |