Table 3.
Rate constants (kobs) for deacylation of Hs aa-tRNAPro by WT and mutant Hs ProXp-ala
Deacylation assays were performed at 30 °C using 1.5 μm Hs ProXp-ala and 100 nm Hs aa-tRNAPro. With the exception of Pro-tRNAPro deacylation by Hs ProXp-ala M35G (one trial), all results are the averages of three independent trials with the standard deviation indicated.
| Ala-tRNAPro |
Ser-tRNAPro |
Pro-tRNAPro |
|||
|---|---|---|---|---|---|
| kobs | kobs | Fold change (Ala/Ser) | kobs | Fold change (Ala/Pro) | |
| min−1 | min−1 | min−1 | |||
| WT | 0.79 ± 0.12 | 0.039 ± 0.003 | 20 | 0.16 ± 0.012 | 5.0 |
| M35G | 0.17 ± 0.008 | 0.089 ± 0.011 | 1.9 | 0.029 | 5.9 |
| H137A | 0.80 ± 0.019 | 0.045 ± 0.004 | 18 | 0.38 ± 0.056 | 2.1 |