Figure 5.

mTPP uncovers thermal destabilization of all 20S core subunits of the proteasome in pup2-ts mutant cells. A and B, representative raw data melt curves from WT versus pup2-ts for each of the 14 subunits of the 20S proteasome core (A) and the 19 subunits of the 19S regulatory particle (B) derived from data from the mTPP experiment. Each line represents an individual subunit. C and D, individual normalized melt curves of representative proteins Pre10 from the 20S core (C) and Rpn2 from the 19S regulatory particle (D).