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. 2020 Oct 31;39(23):e105364. doi: 10.15252/embj.2020105364

Figure EV5. Aminoacylation assay in fibroblasts.

Figure EV5

Aminoacylation assay of mt‐tRNAGlu and mt‐tRNAGln in fibroblasts carrying digenic mutations in mtDNA (m.14674T>C) and in EARS2 (p.Arg516Gln; F7/1M, F7/2M), carrying only m.14674T>C (F7/5F, F7/7F) and healthy controls. The cells were cultures in low (low aa) and high amino acid (aa) concentrations. “dAc” indicated deacylated samples while “Ac” represents the aminoacylated tRNA. There is no relevant difference in the aminoacylation of fibroblasts of patients with digenic mutations, carriers of m.14674T>C and healthy controls.