Table 2.
Changes in the mean fluorescence intensity of surface multipotent mesenchymal stromal cells untreated and treated with interleukin 1β, interferon gamma and tumour necrosis factor alpha antigens
|
MFI
| ||||||||
|
|
MSCs
|
MSCs treated with IL1β |
MSCs treated with IFN
|
MSCs treated with TNF
|
||||
|
Antigen
|
Effective
|
Ineffective
|
Effective
|
Ineffective
|
Effective
|
Ineffective
|
Effective
|
Ineffective
|
| HLA-ABC | 14561 ± 7741 | 8767 ± 1434 | 14740 ± 10211 | 8850 ± 994 | 15200 ± 9601 | 10208 ± 1189 | 21174 ± 1615 | 15706 ± 2938 |
| HLA-DR | 752 ± 1501 | 188 ± 42 | 610 ± 1541 | 149 ± 25 | 1077 ± 1531 | 423 ± 163 | 659 ± 1371 | 169 ± 38 |
| CD54 | 4881 ± 371 | 4447 ± 1203 | 13773 ± 32181 | 4234 ± 1612 | 7889 ± 8161 | 4378 ± 1566 | 61708 ± 5998 | 65461 ± 11324 |
| CD73 | 3047 ± 253 | 2354 ± 343 | 3396 ± 305 | 2226 ± 543 | 3414 ± 383 | 2678 ± 655 | 3395 ± 305 | 3370 ± 605 |
| CD90 | 57679 ± 2626 | 49241 ± 7022 | 60117 ± 3990 | 45739 ± 9525 | 56500 ± 30721 | 40335 ± 4502 | 55799 ± 3471 | 45198 ± 7142 |
| CD105 | 35682 ± 26231 | 21471 ± 3247 | 34402 ± 2490 | 29665 ± 6032 | 41171 ± 3118 | 30525 ± 5609 | 30431 ± 2471 | 24260 ± 3466 |
| CD146 | 4345 ± 5341 | 1807 ± 482 | 3546 ± 497 | 3124 ± 688 | 3762 ± 479 | 2378 ± 707 | 3137 ± 387 | 3463 ± 539 |
| CD274 | 293 ± 37 | 261 ± 38 | 227 ± 204 | 400 ± 104 | 304 ± 219 | 341 ± 54 | 322 ± 81 | |
| %CD54 | 56 ± 3 | 50 ± 9 | 57 ± 4 | 42 ± 8 | 66 ± 3 | 55 ± 8 | 89 ± 2 | 93 ± 4 |
1
Significant differences between effective and ineffective samples. MFI: Mean fluorescence intensity; MSC: Multipotent mesenchymal stromal cell; IL1β: Interleukin 1β; IFN: Interferon gamma; TNF: Tumour necrosis factor alpha.