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. 2020 Nov 30;11:6087. doi: 10.1038/s41467-020-19919-y

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a Control (Ctrl-D10 (filled circle) and Ctrl-I004 (filled triangle)) and MINPP1 LOF (patient-derived (CerID-30-2 (blank circle) and MINPP1^−/− (blank triangle)) iPSCs were differentiated toward neuronal lineage for 14 days. Representative images of the differentiated cells stained with early neuronal marker TUJ1 and neural progenitor marker PAX6. Hoechst was used as a nuclear stain. b Quantitative analysis of the immunofluorescence data. c Representative images of TUNEL staining during neuronal differentiation at day 14. d Quantification of the TUNEL assay during neuronal differentiation (iPSC, day 10 and day 14). All scale bars correspond to 50 μm. (For (b, c), n = 4, for (d), n = 6 for iPSCs and day 14, n = 4 for day 10. Duplicate/triplicate analysis of two independent experiments. Two-tailed student’s t-test, **p ≤ 0.01 and ***p ≤ 0.001. For (b) upper panel, Ctrl vs. MINPP1 LOF: p = 0.0001; for (b) lower panel, Ctrl vs. MINPP1 LOF: p = 0.0066; for day 10 data (d), Ctrl vs. MINPP1 LOF: p = 0.0045 and for day 14 data (d), Ctrl vs. MINPP1 LOF: p = 0.0003). The data are presented as mean percentage values ± s.d. For (b) and (d), source data are provided as a source data file.