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. 2020 Nov 30;11:6087. doi: 10.1038/s41467-020-19919-y

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a–d SAX-HPLC analysis of inositol phosphate levels in MINPP1^−/− HEK293 cells (a), patient fibroblasts (CerID-30-1 and CerID-30-2) (b), MINPP1 LOF (patient-derived (CerID-30-2 (blank circle) and MINPP1^−/− (blank triangle)) iPSCs (c), and their day-10 differentiating neuron counterparts (d). The peaks ([³H]-IP_n) were identified based on comparison to standards. [³H]-IP_n levels are presented as percentage of total radioactivity in the inositol-lipid fraction ([³H]-PIP_n). All error bars represent standard deviation (s.d.). (For (a), n = 2 and both experiments are represented. For (b–d), n = 4, two-tailed student’s t-test, *p ≤ 0.05 and **p ≤ 0.01). IP_n, inositol phosphates; PIP_n, phosphatidylinositol phosphates. Source data are provided as a source data file.