Fig. 2. Intraganglionic injections of AAV5-hSyn-hM3Dq primarily transduces large caliber DRGs.

a–i AAV5-hSyn-hM3Dq was injected into the right C5-C8 DRGs. Four weeks later, the DRGs were isolated and immunostained for mCherry (a, d, g, red) and calcitonin gene related peptide (CGRP; b, blue), IB4 (e, blue) or NF-200 (h, blue). We observed mCherry expression mostly in large diameter, NF-200⁺ neurons (g–i) rather than nociceptive CGRP⁺ (a–c) or IB4⁺ neurons (d–f). j Quantification of the transduction rates in CGRP⁺, IB4⁺ and NF-200⁺ neurons demonstrates that AAV5 transduces NF-200⁺ neurons more efficiently than smaller caliber, nociceptive neurons. N = 12 animals. Mean ± SEM. One-way ANOVA and post-hoc multiple comparisons testing using the two-stage step-up method of Benjamini, Krieger, and Yekutieli, ****p < 0.0001. Scale bar: 100 µm. Source data are provided as a Source Data file.