Table 3.

Animal species detected by DNA metabarcoding.

Species name	G-1
	Number of sequence reads	Number of OTUs
Primer: gSalamander
Hynobius retardatus	61,428	4
Gadus chalcogrammus (used as bait)	15,636	4
Procyon lotor	1,453	1
Total	78,517	9

Species name	G-1		G2		S-2
	Number of sequence reads	Number of OTUs	Number of sequence reads	Number of OTUs	Number of sequence reads	Number of OTUs
Primer: gInsect
Yezoterpnosia nigricosta	0	0	0	0	26,844	31
Curculio davidi	35,735	7	0	0	0	0
Chironomidae	15,252	6	0	0	0	0
Eubasilissa regina	0	0	6,302	2	0	0
Procyon lotor	0	0	505	1	0	0
Total	50,987	13	6,807	3	26,844	31

Species name	G-1		G2		S-2		S-3
	Number of sequence reads	Number of OTUs	Number of sequence reads	Number of OTUs	Number of sequence reads	Number of OTUs	Number of sequence reads	Number of OTUs
Primer: COI
Hynobius retardatus	1,337	1	0	0	0	0	0	0
Rana pirica	365	1	0	0	0	0	0	0
Yezoterpnosia nigricosta	0	0	0	0	14,955	34	0	0
Armadillidium vulgare	0	0	0	0	0	0	505	1
Curculio sikkimensis	491	1	0	0	0	0	0	0
Eubasilissa regina	0	0	16,320	3	0	0	149	1
Lelia decempunctata	109	1	0	0	0	0	0	0
Limnophyes minimus	415	1	0	0	0	0	0	0
Megascolecidae sp.	282	1	0	0	0	0	417	1
Euhadra brandtii	535	1	415	1	0	0	0	0
Ezohelix gainesi	0	0	388	1	0	0	0	0
Other species (Fungi etc.)	2,884	4	244	2	1,153	3	7,518	10
Other species (Fish and meat used as bait)	1,994	2	0	0	0	0	411	1
Procyon lotor	846	1	2,191	1	0	0	758	1
Total	9,258	14	19,558	8	16,108	37	9,758	15