Figure 1.

Experimental design. Mice were acclimated to powdered food and CLAMS chambers beginning 12 days prior to mating. Seven days prior to mating mice were randomly assigned to either CD or HFHS. On day − 1 mice were mated with proven breeder males for one night, mice where a copulatory plug was detected were considered pregnant and those where no copulatory plug was detected were designated non-pregnant. The day of copulatory plug detection (day 0) mice were placed in CLAMS cages for EE, food intake, and activity level measurements until day 17.5 pregnancy. At days 0, 6. 5, 10.5, 13.5 and 17.5 mice were briefly taken out of the cages, weighed, cages were cleaned, and then re-sealed. On day 17.5 mice were sacrificed and VAT was collected for further experiments.