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. 2020 Nov 30;10:20866. doi: 10.1038/s41598-020-77529-6

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Brief HFHS diet exposure results increased energy exposure (EE). EE (A), locomotor activity (B), and food intake (C) were measured from day 1.5 through day 17.5 of pregnancy in NP-CD, P-CD, NP-HFHS, and P-HFHS dams. For EE (A) there was a significant interaction of diet*pregnancy*day (p = 0.0015). Activity (B) was significantly decreased in P vs NP females (p < 0.0001) and in HFHS vs CD fed females (p < 0.01). For food intake there was an interaction of diet*pregnancy (p < 0.0007). n = 7(NP-CD), 9 (P-CD), 11 (NP-HFHS) and 5 (P-HFHS); error bars ± SEM.