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. 2020 Nov 30;10:20866. doi: 10.1038/s41598-020-77529-6

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Analysis of Adm signaling in P-CD and P-HFHS Dams. Adm signaling is increased in VAT from GDM dams at day 17.5 of pregnancy. VAT mRNA expression of (A) Adm, (B) Crlr, (C) Ramp2, and (D) Ramp3. (E) Glycerol release was measured in ex vivo culture media of visceral fat from P-CD and P-HFHS dams w/o Adm after 24hrs as a measure lipolysis. Adm increased lipolysis in a dose dependent manner in both P-CD and P-GDM dams. *p < 0.05, different subscripts represent differences among groups (p < 0.01); n = 6 P-CD and 5 P-HFHS, error bars ± SEM.