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. 2020 Dec 1;7:200. doi: 10.1038/s41438-020-00418-6

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a CCAAT-box distribution within the SlGME1 2-kb promoter. There are four cis-elements in the 2-kb promoter region of SlGME1. Four CCAAT elements are represented by black boxes and are named C1, C2, C3, and C4. b Dual-luciferase assay between the SlGME1 promoter and SlNFYA10. The full sequence and serial truncated versions of the SlGME1 promoter were ligated into pGreenII 0800-LUC as a reporter vector, and SlNFYA10 was ligated into pGreenII 62-SK as an effector vector. PG, the pGreenII 62-SK empty vector with serial SlGME1 truncated promoters, was used as a control. LUC luciferase activity. RLC Renilla luciferase. The data are means ± SEs (N = 3)