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. 2020 Dec 1;7:200. doi: 10.1038/s41438-020-00418-6

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a The SlGME1 promoter fragment harboring C1 and C2 elements interacts with SlNFYA10 according to a yeast one-hybrid assay. The C1 and C2 elements along with their mutated version constituted four combinations (the first contained native C1 and C2 elements, the second contained mutated C1 and native C2 elements, the third contained native C1 elements and mutated C2 elements, and the fourth contained mutated C1 and C2 elements). These promoter segments were inserted into the pAbAi vector as bait, and SlNFYA10 was inserted into the pGADT7 vector as prey. The bait and prey vectors were cotransformed together along with a negative control (bait-pGADT7) or positive control (p53-AbAi/pGAD-p53) into yeast on selective media with or without antibiotics (25 ng/ml AbA). a The interaction between C2 elements and serial SlNFYA10 domains according to a yeast one-hybrid assay. On the basis of its structural characteristics, SlNFYA10 was divided into four segments and inserted into the pGADT7 vector to serve as prey, and the SlGME1 promoter contained the whole C2 element as bait. The transformed yeast cells were grown on selective media with or without antibiotics (25 ng/ml AbA)