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. 2020 Nov 30;10:20835. doi: 10.1038/s41598-020-77831-3

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Cage bedding affects body parameters in dietary restricted mice. The amount of bedding consumed by mice submitted caloric restriction (CR) was measured daily between days 11 and 13 of the CR (a). Bodyweight changes were recorded for ad libitum, CR mice as well as over-night (ON) fasted mice and expressed as % change (b). Stomach (c) and cecum (d) weight were measured. Total (e) and active (f) ghrelin concentrations were analyzed in the mice plasma. Oral glucose tolerance test (OGTT) was performed after ON fasting (g). CR mice feces were collected and its weight (h), as well as energy content (i), was assessed. One-way ANOVA was applied to verify statistical significance. Asterisk (*) indicates statistical significance between the indicated groups after Bonferroni correction for multiple testing. The bars indicate the mean of eight to ten biological replicates ± SEM.