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. 2020 Nov 30;10:20835. doi: 10.1038/s41598-020-77831-3

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Cage bedding impacts the composition of cecal microbiota. The composition of bacterial families (a) and microbial diversity (b) in the cecum was analyzed in ad libitum and CR fed mice. The data was presented as a heatmap of the hierarchical clustering analysis of bacterial families using COVAIN (c). The abundance of bacteria in the cecum was expressed as % (d–k). Asterisk (*) indicates statistical significance after Bonferroni correction for multiple testing. Groups were compared using one-way ANOVA. Error bars stand for the mean ± SEM. The data represents nine to ten biological replicates per experimental group.