Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Nov 30;10:20869. doi: 10.1038/s41598-020-77842-0

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

In vitro engineering of adhesive, scaffold-free, 3D hyaline-like cartilage tissue from human bone marrow-derived mesenchymal stem cell (hBMSC) sheets. Our approach uses cell sheet tissue engineering to prepare scaffold-free 3D MSC cellular constructs via spontaneous post-detachment cell sheet contraction from temperature-responsive culture dishes (TRCD). This technology allows in vitro differentiation to hyaline-like cartilage phenotypes by re-plating 3D contracted sheets to cell culture inserts and inducing with chondrogenic media for 3 weeks. Direct adhesion of sheets to target tissue post-differentiation is also possible without damaging the structure or chondrogenic characteristics of the sheet construct. (Created with BioRender.com).