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. 2020 Nov 30;10:20073. doi: 10.1038/s41598-020-77094-y

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Top 5,000 genes with high FPKM value in male (ZZ) and female (ZW) at the following four stages: embryonic gonads at HH27, HH31, and HH38, and the adult testis or ovary. Bar graphs were showed separately for autosomes (left side) or the Z chromosome (right side). Genes were classified into the following four groups according to fold change between sexes at the same stage: unbiased (grey), × 1.5–2.0 fold change (light blue for male and light orange for female, respectively), × 2.0–3.0 (blue and orange), and × 3.0- (dark blue and brown).