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. 2020 Nov 30;10:20073. doi: 10.1038/s41598-020-77094-y

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Expression patterns of nine clusters during gonadal sex differentiation. Between HH27 and HH31, gene expression levels increased in clusters 1, 2 and 3, remained constant in clusters 4, 5 and 6, and decreased in clusters 7, 8 and 9. Similarly, between HH31 and HH38, gene expression levels increased in clusters 1, 4 and 7, remained the same in clusters 2, 5 and 8, and decreased in clusters 3, 6 and 9. Blue and orange lines indicate genes related to gonadal differentiation in males (ZZ) and females (ZW), respectively.