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. 2020 Nov 30;10:20073. doi: 10.1038/s41598-020-77094-y

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Comparison of expression patterns of seven genes between male (ZZ) and female (ZW) gonads using TMM normalized FPKM values. The expression patterns of DMRT1 (A), SOX9 (B), AMH (C), HEMGN (D), NR5A1 (E), FOXL2 (F) and CYP19A1 (G) are shown based on TMM normalized FPKM values in embryonic gonads at HH27, HH31, HH38 and the adult testis or ovary. Blue and orange bars indicate males (ZZ) and females (ZW), respectively.