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. 2020 Nov 30;10:20837. doi: 10.1038/s41598-020-77738-z

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Monitoring of the L. monocytogenes response to osmotic stress with the P_lmo2230::egfp reporter fusion. (A) SigB activity measured as expression of the reporter fusion P_lmo2230::egfp, engineered from the SigB-regulated gene lmo2230. Reporter expression was measured after 30 min incubation in BHI medium containing 0.5 M NaCl. Shown are data representative of a total of three biological replicates. (B) Mean fluorescence intensity quantified in wild-type and DsigB strains in unstress and stress conditions. ***, P < 0.0005 (one-way ANOVA with Bonferroni’s multiple comparisons test). (C) Quantification of the proportion of the bacterial population positive for eGFP levels in the indicated conditions.