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. 2020 Nov 30;12:33. doi: 10.1038/s41368-020-00100-6

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — Characterization of extracellular vesicles (EVs) derived from macrophages seeded on mineralized collagen in vitro. a TEM morphology of extracellular vesicles. b Corresponding particle size and distribution analysis of the samples in a. c Semiquantification of extracellular vesicle diameter. d Western blotting of EV surface markers. e (i) AFM phase images of EVs; (ii) Corresponding modulus mapping images of EVs; (iii) Semiquantification of EV height; (iv) Semiquantification of EV modulus. Arrows: EVs; Ctrl: normal macrophage-derived sEVs. *P < 0.05 vs Ctrl, ***P < 0.001 vs Ctrl, ^#P < 0.05 vs EMC