. 2020 Mar 27;22(6):569–577. doi: 10.4103/aja.aja_11_20

Supplementary Table 3.

Identification of sumoylated proteins in human seminoma

RNA/DNA binding, transcription, translation	Accession number	MW	Number of peptides seminoma	Mouse testis

				Cytes/tids	spg	C18
Heterogeneous nuclear ribonucleoproteins A2/B1	gi\|14043072 (+1)	37 kDa	9	X		X
Heterogeneous nuclear ribonucleoprotein A3	gi\|34740329 [4]	40 kDa	7	X
Heterogeneous nuclear ribonucleoprotein K	gi\|119583080 (+11)	51 kDa	3
Heterogeneous nuclear ribonucleoprotein M	gi\|119589327 (+6)	78 kDa	5
Heterogeneous nuclear ribonucleoprotein H2	gi\|9624998	49 kDa	2	X
Heterogeneous nuclear ribonucleoprotein -E1	gi\|460771 (+1)	38 kDa	2
Heterogeneous nuclear ribonucleoprotein -F protein	gi\|16876910 [3]	46 kDa	3
Heterogeneous nuclear ribonucleoproteins C1/C2	gi\|117189975 [14]	34 kDa	3
Small nuclear ribonucleoprotein Sm D2	gi\|237649049 (+1)	12 kDa	2	X
Polyadenylate-binding protein 1	gi\|41386798	71 kDa	15
Tat binding protein 1, TBP-1=transcriptional activator	gi\|263098 (+6)	49 kDa	2
Elongation factor 1-delta isoform 2	gi\|25453472 [3]	31 kDa	6	X	X
Elongation factor 2	gi\|4503483	95 kDa	10	X
Elongation factor 1 gamma	gi\|119594432 (+2)	48 kDa	4
Fus-like protein, partial	gi\|1040970 (+7)	53 kDa	4	X
Ewing sarcoma breakpoint region 1	gi\|119580187 (+9)	47 kDa	4
Eukaryotic initiation factor 4A-I	gi\|4503529 [4]	46 kDa	7
Transcription activator SMARCA4/BRG1	gi\|192807314 [12]	182 kDa	4	X
AT-rich interactive domain-containing protein 1A	gi\|21264565 [2]	242 kDa	4	X
Rna Polymerase II	gi\|353251633 [3]	163 kDa	7	X
splicing factor proline/glutamine-rich	gi\|119627826 [4]	66 kDa	5	X
DNA topoisomerase 2-alpha isoform X1	gi\|530412779	173 kDa	4	X
TAR DNA binding protein, TDP43	gi\|119592079 [9]	34 kDa	2	X
Far upstream element-binding protein 2	gi\|154355000 (+4)	73 kDa	2	X
FUSE binding protein 1	gi\|119626762 (+8)	69 kDa
Guanine nucleotide binding protein (G protein)	gi\|119574084	40 kDa	3
Vigilin isoform a	gi\|4885409 (+3)	141 kDa	5
ATP-dependent RNA helicase DDX5	gi\|197692465 [5]	69 kDa
Nuclear receptor coactivator 6 isoform 1	gi\|32307128 (+8)	219 kDa	3
MCM4 minichromosome maintenance deficient 4	gi\|119607091 (+6)	96 kDa	2
Nucleolin	gi\|119591368 (+5)	59 kDa	2
Proliferating cell nuclear antigen	gi\|4505641 (+2)	29 kDa	2
ADP-ribosylation factor 3 isoform X2	gi\|578823704	16 kDa	2			X
14-3-3 protein zeta/delta	gi\|4507953 [6]	28 kDa	10			X
Nm23 Human Nucleoside Diphosphate Kinase B	gi\|1421609 [8]	17 kDa	4			X
Prohibitin isoform 1	gi\|4505773 (+2)	30 kDa	4	X	X
Prohibitin-2 isoform 3	gi\|390608669	29 kDa	2	X		X
Ribosomal protein S4, X-linked	gi\|119592221 [6]	43 kDa	3
Ribosomal protein S16	gi\|119577297 (+1)	16 kDa	3
Ribosomal protein S3	gi\|15718687 (+3)	27 kDa	4
Ras-related protein Rab-1B	gi\|13569962 [2]	22 kDa	2			X
60S ribosomal protein L12	gi\|4506597	18 kDa	3
40S ribosomal protein S7	gi\|4506741	22 kDa	3
Ribosomal protein L7	gi\|307388	29 kDa	4
60S ribosomal protein L27	gi\|4506623 (+1)	16 kDa	2
Ribosomal protein L7a	gi\|119608472	33 kDa	3
Ribosomal protein SA	gi\|119584991 (+5)	20 kDa	4
Ribosomal protein L23a	gi\|119571516 (+3)	22 kDa	2	X
Ribosomal protein S10	gi\|119624187 (+3)	20 kDa	3
40S ribosomal protein S9	gi\|14141193 [2]	23 kDa	3			X
Ribosomal protein L21 variant	gi\|62087780	19 kDa	2	X
Ribosomal protein, large, P0	gi\|12654583 (+4)	34 kDa	4
40S ribosomal protein S20 isoform 1	gi\|226246671 (+1)	16 kDa	3
60S ribosomal protein L11 isoform 2	gi\|315221152 (+1)	20 kDa	2	x
Ribosomal protein S19	gi\|119577478 (+2)	17 kDa	3	x
Similar to ribosomal protein L23	gi\|13097600 (+2)	14 kDa	4	x
60S ribosomal protein L31 isoform 2	gi\|148746199 (+6)	15 kDa	3
40S ribosomal protein S13	gi\|4506685	17 kDa	3
60S ribosomal protein L26	gi\|4506621 (+1)	17 kDa	4	x
60S ribosomal protein L10a	gi\|4506621 (+1)	17 kDa	4	x		x
Ribosomal protein S15a ribosomal protein S3A 40S ribosomal protein S14	gi\|12804561 (+5) gi\|119625394[8] gi\|5032051	15 kDa 28 kDa 16 kDa	3 2	x
60S ribosomal protein L24	gi\|4506619	18 kDa	2
40S ribosomal protein S2-like isoform 5	gi\|114647215 [3]	31 kDa	2
Ribosomal protein S6	gi\|337514	29 kDa	2
Ribosomal protein S26	gi\|119617283 (+5)	12 kDa	2	x
Ribosomal protein S5	gi\|119592989 (+2)	22 kDa	2
Ribosomal protein L30	gi\|119612175 [2]	10 kDa	2
Cytoskeleton
Plectin isoform 1a	gi\|41322923 (+1)	516 kDa	13			X
Actin-related protein 2/3 complex subunit 4	gi\|5031595 (+3)	20 kDa	18			X
Actin-related protein 3 isoform 2	gi\|460417294)	42 kDa	2
Myosin light polypeptide	gi\|113812151 (+6)	16 kDa	2	X
Myosin-9	gi\|12667788	227 kDa	22
Cofilin-1	gi\|5031635	19 kDa	3
Spectrin beta chain, non-erythrocytic 1	gi\|112382250 (+8)	275 kDa	4
Spectrin, alpha, non-erythrocytic 1	gi\|119608216	286 kDa	2
Transgelin 2, isoform CRA_e	gi\|119573148 (+4)	22 kDa	16			X
Talin-2	gi\|344179032 [6]	167 kDa	10	X
Asap3 , ankyrin repeat and PH domain 3	gi\|297787706 (+7)	55 kDa	2
Catenin delta-1 isoform 1A	gi\|146231938 (+21)	104 kDa	2
Filamin-A isoform 1	gi\|116063573 [7]	280 kDa	2
Filamin-B isoform 2	gi\|105990514 [18]	278 kDa	2	X
Lamin A/C	gi\|119573381 (+13)	78 kDa	8
Lamin B1	gi\|15126742 (+1)	66 kDa	12	X	X
IQ motif containing GTPase activating protein 1	gi\|141797011 [3]	189 kDa	2
Dynein, cytoplasmic 1, heavy chain 1	gi\|119602165 (+5)	526 kDa	2			X
Vinculin	gi\|119574932 [6]	109 kDa	3	X
Triple functional domain (PTPRF interacting)	gi\|119628449 (+3)	268 kDa	2
Alpha-centractin isoform 2	gi\|114632563 (+6)	37 kDa	2
Porin 31HM	gi\|238427 (+3)	31 kDa	5
Ubiqituination , proteasomal degradation			10
Valosin-containing protein	gi\|111305821 (+1)	89 kDa	4			X
Ubiquitin B	gi\|119624910	13 kDa	3	X		X
Ubiquitin associated Protein (AD-012 protein)	gi\|7688705	61 kDa	6
Ubiquitin associated protein 2-like	gi\|13111995 (+17)	115 kDa	6	X
26S proteasome subunit p97	gi\|1060888 (+11)	100 kDa	2	X
Cullin-associated NEDD8-dissociated protein 1	gi\|16758920	136 kDa	3
Membrane-associated, vesicle trafficing			5
GPI-anchored membrane protein 1	gi\|119588582 [2]	77 kDa	4	X		X
annexin A1 annexin V chloride intracellular channel protein 1	gi\|4502101 gi\|342350777[2] gi\|14251209 [8]	39 kDa 36 kDa 27 kDa	4 3	X		X
Clathrin heavy chain 1 isoform 1	gi\|4758012 [6]	192 kDa	17
ras-related protein Rab-2A isoform b	gi\|336391093)	21 kDa	3			X
Ras-related protein Rab-7a	gi\|34147513	23 kDa	3	X		X
ras GTPase-activating protein-binding protein 1G3BP	gi\|49168554 [3]	52 kDa	4	X
rab11 In Complex With Rab11-Fip2	gi\|114794171 (+11)	19 kDa	2
ras-related protein Rab-14	gi\|18390323	24 kDa	2	X
rho GDP-dissociation inhibitor 1 isoform b	gi\|297374785 (+3)	18 kDa	2			X
Chaperones/stress-related proteins			10
Heat shock 70kDa protein 4	gi\|119582699 (+3)	88 kDa	4	X		X
Heat shock protein gp96 precursor	gi\|15010550 (+2)	90 kDa	5
Heat shock protein 47, member 1	gi\|119595384 (+4)	41 kDa	2
Chaperonin containing TCP1, subunit 4 (delta)	gi\|119620393 (+2)	58 kDa	3
Chaperonin containing TCP1, subunit 3 (gamma)	gi\|14124984 (+7)	60 kDa	3
Chaperonin containing TCP1 subunit 8KIAA0002	gi\|1136741 (+3)	59 kDa	2
Hypoxia up-regulated protein 1 precursor	gi\|5453832 [4]	111 kDa	2			X
Peroxiredoxin 1, isoform CRA_b	gi\|119627382 (+5)	21 kDa	4
t-complex, isoform CRA b	gi\|119568002 (+3)	35 kDa	2	X
Enzymes
ATP synthase, H+transporting	gi\|119630208 (+3)	17 kDa	2
ATP synthase subunit beta, mitochondrial precursor	gi\|32189394 (+2)	57 kDa	3	X	X	X
ADP/ADT translocator protein	gi\|339920 [2]	33 kDa	10
Triosephosphate isomerase	gi\|441670320	23 kDa	6	X		X
Phosphate carrier protein, mitochondrial isoform b	gi\|4505775 (+5)	40 kDa	3	X
Malate dehydrogenase, mitochondrial isoform 1	gi\|21735621 (+5)	36 kDa	6
Beta-enolase isoform X1	gi\|530410063	48 kDa	2
6-phosphogluconate dehydrogenase	gi\|40068518 [4]	53 kDa	2
Human glutamate oxaloacetate transaminase 1	gi\|255918055 (+3)	48 kDa	2
GAPDH-2 like	gi\|2282013 (+5)	45 kDa	2
Aconitase precursor	gi\|3600098	65 kDa	5
Arginyl-tRNA synthetase	gi\|119581911 (+6)	75 kDa	3
Glutamine-fructose-6-phosphate transaminase 1	gi\|119620257 (+4)	65 kDa	2
Acyl-CoA thioesterase 7	gi\|119591933 (+11)	30 kDa	2
Multifunctional protein CAD	gi\|1228049 (+7)	243 kDa	3
Nuclear-cytoplasmic transport
Karyopherin (importin) beta 1	gi\|119615215 (+3)	94 kDa	4	X		X
Ran GTPase activating protein 1	gi\|119580824 (+6)	72 kDa	3	X	X	X
Exportin-2 isoform 1	gi\|29029559 (+5)	110 kDa	2	X
Immune response
Chain A, Cyclophilin B	gi\|1310882 (+5)	20 kDa	2
Chain B, Traf6 Chain A, The 25 Kda subunit of human cleavage facto	gi\|238538004 (+5) gi\|168177225	26 kDa	2
Expressed only in cancer tissues
TRK-fused gene/anaplastic large cell lymphoma kinas	gi\|20269390 (+1)	89 kDa	2

Protein accession number, MW, and the number of unique peptides identified for each protein (number of peptides) is indicated. Proteins that were also identified as SUMO targets in mouse spermatocytes and/or spermatids, spermatogonia, or C18-4 cells (cytes/tids, spg, C18-4 respectively) are indicated by “X.” Proteins of specific interest are indicated in bold. FUSE: far upstream element; MW: molecular weight