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. 2020 Apr 7;22(6):590–601. doi: 10.4103/aja.aja_3_20

Figure 1.

Figure 1

Analysis of the transcriptomes of the caput epididymidis of aged WT and Gpx5-/- mice. (a) Difference in mRNA expression between the WT and Gpx5-/- groups. Each gene is represented by a dot located according to its expression level between the WT and KO groups. (b) RNA-seq data validation by RT-qPCR of Gpx5 and another three randomly chosen genes in the 13-month-old WT and Gpx5-/- mice. Quantitative PCR data are presented as mean ± s.d. from three independent animals. NS: not significant; *P < 0.05; Student's t-test. (c) GO enrichment result for the up-regulated genes in the caput epididymis of Gpx5-/- mice. (d) GO enrichment result for the down-regulated genes in the caput epididymis of Gpx5-/- mice. RPKM: reads per kilobase of transcript per million mapped reads; GO: gene ontology; RT-qPCR: quantitative reverse transcription PCR; Gpx5: glutathione peroxidase 5; s.d.: standard deviation; WT: wild-type; KO: knockout.