Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Apr 7;22(6):590–601. doi: 10.4103/aja.aja_3_20

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright: ©The Author(s)(2020)

This is an open access journal, and articles are distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as appropriate credit is given and the new creations are licensed under the identical terms.

PMC Copyright notice

Alteration in the mRNA expression of piRNA pathway genes in the caput epididymidis of aged Gpx5^-/- mice. (a) GSEA analysis result of the piRNA pathway genes. A positive ES with a significant P value and FDR indicates higher mRNA levels of piRNA pathway genes in the WT group and the significant downregulation in the Gpx5^-/- mice. (b) RNA-seq data validation by RT-qPCR of six known piRNA pathway genes in the 13-month-old WT and Gpx5^-/- groups. Quantitative PCR data are presented as mean ± s.d. from three independent animals. NS: not significant; ^*P < 0.05; ^**P < 0.01; Student's t-test. GSEA: gene set enrichment analysis; ES: enrichment score; FDR: false discovery rate; RT-qPCR: quantitative reverse transcription PCR; Gpx5: glutathione peroxidase 5; s.d.: standard deviation; WT: wild-type; KO: knockout; NES: normalized enrichment score; piRNA: P-element-induced wimpy testis (PIWI)-interacting RNA.